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How to Prepare and Assess Living Myocardial Slices: A Workshop

How to Prepare and Assess Living Myocardial Slices: A Workshop

Description

Living Myocardial Slices (LMS) are a novel preparation of intermediate complexity that can be used to study heart biology. LMS possess complex 3D structure, heterocellularity and intact extracellular matrix that makes them ideal for functional and structural studies. LMS can be prepared from several species, including human. Multiple slices can be produced from each biopsy, producing several samples for experimentation. LMS are highly viable, stable in culture and can be used for toxicology and pharmacology experiments, to study pathophysiology and treatments for cardiac disease.

While LMS are prepared by a straightforward method - simple mechanical dissociation using a vibrating microtome, several essential details contribute to the quality and viability, and this explains the variability of results in different laboratories. This workshop will discuss LMS in the context of other myocardial in-vitro models, highlighting strengths and limitations of the technique, demonstrating the preparation of this model and offering the participants a hands-on opportunity to learn this technique.

 

Enquiries

Sam Reitemeier ([email protected])

Dates

4 September - 5 September 2024

 


How to Prepare and Assess Living Myocardial Slices - September 2024 Academic rate

£120.00


How to Prepare and Assess Living Myocardial Slices - September 2024 Industry rate

£240.00